Atcc hl-60

HL-60/S4 ATCC ® CRL-3306™ Homo sapiens peripheral blood acut To ATCC Valued Customers, ATCC stands ready to support our customers’ needs during the coronavirus pandemic.

In some experiments, HL-60 cells were treated with Organism Type: Human: This is a non-adherent human leukocyte cell line. ATCC # CCL-240: Tissues: Lymphocyte: Phenotype: Suspension: Primary: no: Application: Cell The settings were as followed: 2*10^6 cells (Hl-60 and Granulocytes) centrifuged and resuspended in 0.5 ml NBT solution (2 mM). Incubated for 30 minutes at 37°C . Stopped the reaction with 2M HCl. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH HL-60 is a suspension cell line (see ATCC on this), so most of the cells stay in the liquid phase and do not adhere to the plastic surface of the culture flask.

07.05.2021

All amounts and volumes are given on a per well basis. 1. The day of transfection, count the cells to determine culture density. Plate 1 x 105 cells per well in 0.5 ml of HL-60 CELL LINE RC2DVQ0F6O Overview Names 3: Identifiers 2: Relationships 2: Characteristic Attributes 3: Notes 2: Audit Info References 3: Substance Class Jan 17, 1989 · Although intact ATCC HL-60 cells specifically and saturably take up 1,25-dihydroxy[3H]vitamin D-3, whole cell extracts have little or no specific binding of 1,25-dihydroxyvitamin D-3. The absence of specific binding can now be explained by the action of a serine proteinase in these cells. Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts.

Eosinophilic cell lines, HL-60 clone 15 cells and EoL-1 cells, have contributed to clarifying the mechanisms responsible for differentiation into eosinophils. These cells differentiate into eosinophils by continuous histone acetylation. Histone deacetylase inhibitors, sodium butyrate and apicidin, p …

Two types of HL-60 cells, 1,25-(OH)2D3-responsive ATCC HL-60 cells and 1,25-(OH)2D3-resistant LG HL-60 cells were used. Despite the presence of enough amounts of normal 1,25-(OH)2D3 receptors, only 22% of LG cells matured after a 4-day treatment with 10(-7) M 1,25-(OH)2D3, while 80% of ATCC cells differentiated. Apr 15, 2010 · HL-60 cells (CCL-240) and U-937 cells (CRL-1593.2) were supplied by American Type Culture Collection (ATCC). HL-60 cultures were maintained at concentrations between 1×10 5 and 1×10 6 of viable cells/ml at 37°C under 5% CO 2 in air and 95% humidity, in Iscove's modified Dulbecco's medium (IMDM) supplemented with 20% heat-inactivated fetal The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA).

There is no reported evidence by the originators of HL-60 (33), the American Type Culture Collection (ATCC) (Rock-ville, Md.), or the European Collection of Cell Cultures

GSE985: Gene Expression-Based High Throughput Screening: HL-60 Cells sapiens provider American Type Culture Collection (ATCC) Sex male cell line 7 ATCC – American Type Culture Collections, Manassas, VA. 8 Centre for Oncology and M14 (human melanoma). 7. HL-60 (human acute myeloid leukemia). center.

A computational study of the open and closed forms of the N-lobe human serum transferrin apoprotein.. PubMed. Rinaldo, David; Field, Martin J. 2003-12-01. Human serum transferrin tightly binds ferric ions in the blood stream but is able to release them in cells by a process involving receptor-mediated endocytosis and decrease in pH. Oct 30, 2017 · We constructed an effective model of ATRA-induced HL-60 differentiation which described signaling and gene expression events following the addition of ATRA (Fig. 1).HL-60 is a NCI-60 cell line HL-60 cells to grow healthy need higher con.

Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte) The human acute promyelocytic leukemia HL-60 (ATCC® CCL-240™), Jurkat (human acute T lymphocytic leukemia, ATCC® TIB-152™) and K-562 (human chronic myelogenous leukemia, ATCC® CCL-243™) suspension cells were maintained in RPMI-1640, while HepG2 (human hepatocellular carcinoma, ATCC® HB-8065™), MCF7 (human breast adenocarcinoma, ATCC model – HL-60 Study example Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type. A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for I usually replenish my stock of HL-60 cells from ATCC when I encounter problems with differentiation. Hope this helps, Paige.

Cite. 10th Sep, 2014. Steven J Ackerman. University of Illinois at HL-60 cells were obtained from Dr Dallas Hyde (University of California, Davis, USA) or ATCC (Manassas, VA, USA) and maintained in RPMI 1640 supplemented with 10% foetal calf serum. Non-differentiated cells were maintained at 1.5x105 to 8x105 cells/ml and were passaged every 2–3 days. In some experiments, HL-60 cells were treated with Organism Type: Human: This is a non-adherent human leukocyte cell line. ATCC # CCL-240: Tissues: Lymphocyte: Phenotype: Suspension: Primary: no: Application: Cell The settings were as followed: 2*10^6 cells (Hl-60 and Granulocytes) centrifuged and resuspended in 0.5 ml NBT solution (2 mM).

34 MOLT-4. 30,000. Leukemia. 27.9.

Both cell lines express monocyte-specific esterase (MSE) and MLL-AF9 fusion m … 1/2/2021 ATCC products for toxicology 6 ATCC is the complete solution supplier for toxicology From basic research through discovery and development to product testing −Continuous cell lines −Primary cells −hTERT-immortalized primary cells −upcyte ® hepatocytes (ATCC ® ACS-9000 ™) Portfolio features −Reliability −Fully characterized cells 1/2/2019 The human acute promyelocytic leukemia HL-60 (ATCC® CCL-240™), Jurkat (human acute T lymphocytic leukemia, ATCC® TIB-152™) and K-562 (human chronic myelogenous leukemia, ATCC® CCL-243™) suspension cells were maintained in RPMI-1640, while HepG2 (human hepatocellular carcinoma, ATCC® HB-8065™), MCF7 (human breast adenocarcinoma, ATCC® … HL-60 is a promyelocytic cell line derived by S.J. Collins, et al.

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The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established.

Nucleofector® II. BJAB Cells · CCRF-CEM Cells · Daudi Cells · F-36P Cells · FO Cells · HL-60 Cells · K562 Cells · LP-1 Cells · M-NFS-60 Cells · MC116 Cells · Mino Cells 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in 19 Apr 2012 22Rv1, 22Rv1, prostate, carcinoma, 22Rv1, M, ATCC, -, -, CVCL_4Y35 · - · - haematopoietic_neoplasm, HL-60, F, ATCC, HL-60, Lymphoid ATCC website. No PMID. Clom 15 ATCC FAQ #11058.